What is mueller hinton agar?

Mueller Hinton agar is a microbiological growth medium used for antibiotic susceptibility testing of bacteria. It was first developed by John Hinton and Rachel Mueller in 1941.

Mueller Hinton agar is composed of beef infusion, casein hydrolysate, and starch. It has a neutral pH, which makes it suitable for the growth of a wide range of bacteria.

The agar is poured into petri dishes and allowed to solidify, providing a stable surface for bacterial growth. Antibiotic discs are then placed on the agar surface, and the bacteria are inoculated onto the plate. After incubation, the zones of inhibition around the antibiotic discs are measured to determine the sensitivity of the bacteria to the antibiotics.

Mueller Hinton agar is widely used in clinical microbiology laboratories to test the susceptibility of bacteria to antibiotics. It is a standardized medium that provides consistent and reproducible results, making it a valuable tool in the fight against antibiotic resistance.